Dr. Arvind Kumar Shakya
Toxicology Research (2022)
Authors: ArvindKumarShakya1,* , NeetuSharma2 , MonikaBhadauria3 , SatendraKumarNirala4 , SadhanaShrivastava5
https://doi.org/10.1093/toxres/tfac052 PolyherbalUnaniformulationshavebeenusedinthetreatmentofliverdiseasesforalongtime.(IbrahimM,KhajaMN,AaraA,KhanAA,HabeebMA,DeviYP,NarasuML,HabibullahCM.HepatoprotectiveactivityofSapindusmukorossiandRheumemodiextracts:invitroandinvivostudies.WorldJGastroenterol.2008:14:2566–2571.)TheaimofthepresentstudywastoinvestigatecomparativehepatoprotectivepotentialofMajoon-e-Dabeed-ul-ward(MD)andSharbat-e-Deenar(SD)againstCCl4inducedsubchronichepatictoxicity.Invivostudy,albinoratsweredividedinto5groups.GroupIwascontrol;GroupIIwasexperimentalcontroltreatedwithCCl4(0.15mL/kg,i.p.for21days);GroupsIII–IVtreatedwithSD(2mL/kg,p.o.)andMD(1,000mg/kg,p.o.)for5daysfollowingCCl4intoxicationasingroup2respectively;andGroupVwaspositivecontroltreatedwithsilymarin(50mg/kg,p.o.).InvitrohepatoprotectiveactivityofSDandMD(25,50,and100μg/mL)wasassessedbySRBassayandflowcytometryanalysis.CCl4exposuresignificantlyelevatedthereleaseofhepaticenzymesi.e.AST,ALT,LDH,andSALPinserumandlipidperoxidationinlivertissuewhichalltheseparameterswerereversedafterSDandMDadministration.Therapyfor5daysalsonormalizedthelevelsofantioxidantenzymesi.e.catalase,SOD,GPx,GR,tissueGSH,andanilinehydroxylaseinCCl4treatedgroup.DNAdamageandhistologicalalterationscausedbyCCl4wererestoredtowardsnormalgroup.InvitrostudyshowedprotectiveeffectofSDandMDagainstCCl4treatedHepG2celllinesandrathepatocytes.TheresultssuggestedthatMDhasasignificanthepatoprotectivepotentialandregulatoryeffectonoxidativestressthanSDagainstCCl4inducedheatotoxicity,andthatthiseffectmayberelatedtoitsantioxidantactivity.
Journal of Applied Pharmaceutical Science (2019)
Authors: Arvind Kumar Shakya
To identify phytochemicals as NS5B inhibitors against viral NS5B polymerase in silico model. The NS5B polymerase is a hepatitis C virus (HCV) protein involved in the HCV replication. HCV infection can cause progressive liver damage.
(2018)
Authors:
Best Oral Research paper presentation in the National Conference held on 13th Feb. 2018
J Tradit Chin Med (2017)
Authors: Arvind Kumar Shakya, Sangeeta Shukla
To investigate the effect of Sharbat- e-Deenar (SD) on acetaminophen (APAP)-induced hepatotoxicity in rat model.
(2016)
Authors:
Third prize for Oral presentation in the National Conference on 16th July, 2016
Exp Toxicol Pathol (2012)
Authors: Shakya AK, Sharma N, Saxena M, Shrivastava S, Shukla S:
The present study was designed to demonstrate the antioxidant and hepatoprotective effect of Majoone- Dabeed-ul-ward, a Unani herbal formulation. The Majoon-e-Dabeed-ul-ward (MD) at the doses of 250, 500 and 1000 mg/kg, p.o. was administered after carbon-tetrachloride (CCl4; 1.5 ml/kg, i.p. once only) intoxication. Treatment with MD at three doses brought the levels of aspartate transaminase, alanine transaminase, albumin and urea in dose dependent manner. Signification reduction was found in TBARS content and restored the level of reduced glutathione, adenosine triphosphatase, and glucose-6- phosphatase in liver. Therapy of MD showed its protective effect on biochemical and histopathological observation at all the three doses in a dose dependent manner. The study conducted showed that MD possesses strong hepatoprotective activity as decrease the hexobarbitone sleep time and improvement in physiological parameter, excretory capacity (BSP retention time) was seen. DPPH and H2O2 scavenging effects indicated its potent antioxidant activities. The results revealed that MD could afford significant dose-dependent protection against CCl4 induced hepatocellular injury.
